The potential and advances of cryo-ET: unravelling structural heterogeneity in situ
Summary
The resolution revolution has made cryo-electron microscopy an increasingly popular technique to study the structural biology of purified macromolecules and complexes. Extraction of these complexes can have a profound effect on their conformation since biomolecules are usually subject to modifications of and interactions with their native environment. Cryo-electron tomography (cryo-ET) presents a powerful approach to examine macromolecules in situ, enabling the high-resolution visualisation of the molecular organisation and localisation within the cell.