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        Analysis of STED Doughnuts generated by a Spatial Light Modulator

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        masterthesis.pdf (4.231Mb)
        MasterThesis.pdf (4.231Mb)
        Publication date
        2015
        Author
        Boer, A.A.J. de
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        Summary
        This paper summarizes the research done for a master thesis at the Molecular Biophysics group, part the of the Physics Department of Utrecht University. Using a custom microscope set- up, we have researched the effects of polarization on the shape of Stimulated Emission-Depletion doughnuts in a high NA objective, simulating an actual STED environment. Additionally, rather than a vortex phase plate, we have used a Spatial Light Modulator to generate the doughnuts. The results are compared to theoretical predictions resulting from simulations.
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        https://studenttheses.uu.nl/handle/20.500.12932/24278
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