View Item 
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of UU Student Theses RepositoryBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

        T-cell recognition of unconventional antigens

        Thumbnail
        View/Open
        Eindrapportage honours program TvB 20-10-14.pdf (595.0Kb)
        Publication date
        2014
        Author
        Berlo, T.A.M. van
        Metadata
        Show full item record
        Summary
        CD1 proteins, expressed on many antigen presenting cells, are capable of binding lipids to form antigen complexes that contact T cell receptors and activate T cells. Invariant T cell populations exist in mycobacterium infected humans that recognize mycobacterial lipids. Other bacteria might also possess lipid antigens that activate T cells. Using an ELISPOT IFN- assay, we determined that lipids from pathogens other than mycobacteria can stimulate human IFN- responses. We generated T cell lines based on binding of CD1b loaded with lipid antigens. Analysis of T cell lines using ELISPOT revealed not only reactivity to CD1b loaded with S. aureus and B. melitensis lipid antigens, but also to untreated CD1b. A shared phospholipid between bacteria and mammalian cells, phosphatidyl glycerol, was determined to be the stimulating antigen. We sequenced the T cell receptor (TCR) from T cell lines using single cell PCR. Our data show that the identified TCR  and  chain genes are TRAV 9-2 in combination with TRBV 6-2. The identified TCR might play an important role in CD1 mediated immunity and potentially also in CD1 mediated autoimmunity, as phosphatidyl glycerol is also recognized as an antigen.
        URI
        https://studenttheses.uu.nl/handle/20.500.12932/18931
        Collections
        • Theses
        Utrecht university logo