dc.rights.license | CC-BY-NC-ND | |
dc.contributor.advisor | van Rhijn, Ildiko | |
dc.contributor.author | Berlo, T.A.M. van | |
dc.date.accessioned | 2014-11-26T18:02:44Z | |
dc.date.available | 2014-11-26T18:02:44Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/18931 | |
dc.description.abstract | CD1 proteins, expressed on many antigen presenting cells, are capable of binding lipids to form antigen complexes that contact T cell receptors and activate T cells. Invariant T cell populations exist in mycobacterium infected humans that recognize mycobacterial lipids. Other bacteria might also possess lipid antigens that activate T cells. Using an ELISPOT IFN- assay, we determined that lipids from pathogens other than mycobacteria can stimulate human IFN- responses. We generated T cell lines based on binding of CD1b loaded with lipid antigens. Analysis of T cell lines using ELISPOT revealed not only reactivity to CD1b loaded with S. aureus and B. melitensis lipid antigens, but also to untreated CD1b. A shared phospholipid between bacteria and mammalian cells, phosphatidyl glycerol, was determined to be the stimulating antigen. We sequenced the T cell receptor (TCR) from T cell lines using single cell PCR. Our data show that the identified TCR and chain genes are TRAV 9-2 in combination with TRBV 6-2. The identified TCR might play an important role in CD1 mediated immunity and potentially also in CD1 mediated autoimmunity, as phosphatidyl glycerol is also recognized as an antigen. | |
dc.description.sponsorship | Utrecht University | |
dc.format.extent | 609302 | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | |
dc.title | T-cell recognition of unconventional antigens | |
dc.type.content | Master Thesis | |
dc.rights.accessrights | Open Access | |
dc.subject.keywords | CD1, T-cell, T-cell receptor, TCR, phosphatidyl glycerol, lipids, bacteria, Staphylococcus aureus, Brucella melitensis | |
dc.subject.courseuu | Gezondheidszorg landbouwhuisdieren en vet. volksgezondheid | |