View Item 
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of UU Student Theses RepositoryBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

        The influence of oxygen concentration on canine oocyte nuclear maturation in vitro.

        Thumbnail
        View/Open
        The influence of oxygen concentration on canine oocyte maturation in vitro.docx (1.170Mb)
        Publication date
        2013
        Author
        Demuynck, S.
        Metadata
        Show full item record
        Summary
        In this study the influence of oxygen concentration on the oocyte maturation in dogs was investigated. Ovaria were collected from 16 bitches undergoing elective ovariectomy and the cumulus oocyte complexes (COC) were collected. COC’s were matured in synthetic oviductal fluid (SOF) supplemented with luteinizing hormone (LH), follicle stimulating hormone (FSH) and progesterone (P4). 85 oocytes were cultured in group 1 in a humidified environment at 38.7°C, 5% CO2, 7% O2. 88 oocytes were cultured in group 2 in a humidified environment at 38.7°C, 5% CO2, 20% O2. After 72 hours of culture oocytes were stained with Hoechst 33342 and the nuclear maturation stage was determined. Oocytes were classified as germinal vesicle (GV), germinal vesicle breakdown (GVDB), metaphase I (MI), metaphase II (MII), degenerated (D) and not definable (ND). After 72h of culture, the percentages of oocytes still at the GV stage did not differ between both groups (7% O2, 20% GV vs 20% O2, 22% GV). However, the percentage of oocytes that had matured up to the MII stage was significantly (p<0.05) higher after culture in 7% O2 compared to culture 20% O2 (7.7% vs 0% respectively). It is concluded that maturation of canine oocytes in a low oxygen environment is beneficial for nuclear maturation.
        URI
        https://studenttheses.uu.nl/handle/20.500.12932/15990
        Collections
        • Theses
        Utrecht university logo