Data analysis of spectral images for nonlinear optical biopsy

Abstract

This paper summarizes the research done for a bachelor thesis at the Molecular Biophysics department of Utrecht University. In the research, the tumorous skin of hairless mice will be scanned in vivo by a microendoscope to generate Two Photon Fluorescence- and Second Harmonic Generation spectral images. The images will then be analyzed to determine whether they can be used to discriminate between healthy and tumorous tissue. The setup is powered by a ultrashort pulse (140 fs) Titanium Sapphire Laser.