Development of an in vitro model to study digital dermatitis in cows

Abstract

Digital dermatitis (DD) is a common and significant condition in dairy cattle, characterized by painful skin lesions, reduced productivity, and substantial economic losses. Despite decades of research, the aetiology and pathogenesis of DD remain incompletely understood. This study aimed to develop an in vitro model of bovine foot skin to reduce reliance on live animal studies and facilitate research into DD pathogenesis and host-pathogen interactions. Therefore, three studies were conducted i.e. development of a bovine skin culture model, culturing of bacteria involved in the pathogenesis and development of an infection model. Skin Culture Protocols were established for the in vitro culture of bovine foot skin explants. Adult skin remained viable for up to 72 hours, while juvenile skin de