Histological analysis of Static Cold Storage vs Hypothermic Machine Perfusion in porcine hearts
Summary
Heart failure is a growing health issue and a frequent cause of mortality worldwide. Heart
transplantation is the current gold standard to treat people that suffer from end-stage heart disease.
Even though there has been significant progress in the field of heart transplantation over the last
45 years, there are still important challenges and obstacles facing the field, which limit the
application of heart transplantation, including donor organ shortage. In this sense, the optimization
of the process is crucial for medical practice. Currently, static cold storage (SCS) is the clinical
standard for heart preservation. However, there is a number of factors that can complicate the
procedure, including myocardial ischemia injury. On the other hand, hypothermic machine
perfusion (HMP) is considered an ideal approach to extend the donor pool and increase the
utilization rate. In the present study, we sought to investigate the effects of hypothermic machine
perfusion vs static cold storage in slaughterhouse porcine hearts, in order to compare the two
preservation strategies and therefore enhance the existing knowledge, regarding the histological
changes that happen in these hearts during preservation. In this context, we used apical biopsies
from porcine hearts that were preserved for 4 hours with SCS or HMP, and subsequent 4 hours of
normothermic reperfusion to evaluate each method. H&E and PTAH staining were performed to
assess the myocardial injury and mitochondrial damage. Altogether, this approach could not give
us a comprehensive overview of the effect of HMP vs SCS on tissue integrity. Future studies are
essential to further elucidate the effects of machine perfusion on porcine hearts.
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