Structure based calculation of anisotropy in homo-FRET

Abstract

In this thesis the influence of a structure based method on anisotropy calculation of a biological membrane will be discussed. A simulation of a homo-FRET microscopy experiment was made in which a simple biological cell is approximated. This was used to compare the effects of different gridsizes of a structure used to analyse the cell, as well as different intervals of the individual grids and the influence of the inner cell. This was done on misaligned images to see if this method could omit the need for perfect image registration in the experiment. While the method does seem promising, it does invoke a small deviation with respect to the pixel by pixel calculation. This might be solved by a better treatment of the G-factor of the setup in the simulation.