Principle of Focus Shift for a Microscope Objective
Summary
In this research I investigated an optical system of a microscope objective and an imaging lens. This project is a small part of the project: ``Integrated super-resolution correlative microscopy". The main goal for this project is to build a fluorescence wide-field microscope with photo-activated localization microscopy (PALM) capability inside a commercial transmission electron microscope (TEM). In order to gain room inside the limited space of the TEM column, I need to know how to optimize the optical system. I have found formulas which predict the position of the objective relative to the sample and the relative magnification (and therefore magnification).