View Item 
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of UU Student Theses RepositoryBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

        Histological and immunohistochemical characterization of feline hepatic lipidosis.

        Thumbnail
        View/Open
        hepatic lipidosis research project.pdf (1.682Mb)
        Publication date
        2014
        Author
        Gelens, T.
        Metadata
        Show full item record
        Summary
        Feline hepatic lipidosis is an acute critical syndrome that can result into mortality. Classically this condition is considered to involve merely simple steatosis and not progress into liver failure. During histological evaluation some features of steatitis appear to be present. In human medicine there is a condition called non alcoholic fatty liver disease(NAFLD) involving steatosis, steatitis and cirrhosis, to which feline lipidosis may exhibit similarities. To further investigate feline lipidosis we performed immunohistochemistry on samples that have been histologically graded by the NAS-score, used for evaluation and staging of NAFLD. Fifteen archival samples of cat liver have been included in this study, of which twelve feline lipidosis samples and three healthy control animals. All feline lipidosis samples have been scored for steatosis, ballooning, inflammation, fibrosis and oval cells resulting in a NAS-score. Samples have been evaluated by immunohistochemical staining for regeneration of hepatocytes or liver progenitor cells(LPC) (Ki67, K19), inflammatory reaction with macrophages and lymphocytes (MAC387, CD3), hepatic stellate cells(HSC) and fibrosis (αSMA, Sirius red). Feline lipidosis samples show little to no hepatocyte regeneration, little LPC activation and decreased numbers of macrophages and lymphocytes. In all feline lipidosis samples HSC are increased and early fibrosis is present.
        URI
        https://studenttheses.uu.nl/handle/20.500.12932/16574
        Collections
        • Theses
        Utrecht university logo