View Item 
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        •   Utrecht University Student Theses Repository Home
        • UU Theses Repository
        • Theses
        • View Item
        JavaScript is disabled for your browser. Some features of this site may not work without it.

        Browse

        All of UU Student Theses RepositoryBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

        Determining differential expression of Splice Variants in RNA-seq

        Thumbnail
        View/Open
        Weistra - 2012 - Differential expression of splice variants.pdf (957.5Kb)
        Publication date
        2012
        Author
        Weistra, W.L.
        Metadata
        Show full item record
        Summary
        To determine differential expression between different genes, different samples and different experiments quantified data should be normalized using a uniform method. Many protocols and algorithms have been developed over the last years to analyze RNA-seq data, although the field is still in its early days of standardization. A complicating factor in RNA-seq analysis is the existence of genes with multiple splice variants. These variants complicate the task of assigning reads to unique transcripts. In this thesis we will give an overview of the tools and methods available in the literature for analyzing RNA-seq data including differential expression of splice variants for organisms that have a reference genome. In recent years great advances have been made in analyzing the differential expression of alternatively spliced transcripts. For all four steps of the analysis path there are suitable tools available to address the problems alternative splicing causes.
        URI
        https://studenttheses.uu.nl/handle/20.500.12932/11859
        Collections
        • Theses
        Utrecht university logo