dc.rights.license | CC-BY-NC-ND | |
dc.contributor.advisor | Hermens, J.L.M. | |
dc.contributor.author | Oosterwijk, M.T.T. | |
dc.date.accessioned | 2010-07-22T17:00:59Z | |
dc.date.available | 2010-07-22 | |
dc.date.available | 2010-07-22T17:00:59Z | |
dc.date.issued | 2010 | |
dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/4841 | |
dc.description.abstract | Issues concerning free and nominal concentrations of toxic compounds in vitro and in
vivo are widely recognised. When data from in vitro experiments is extrapolated to in
vivo situations it has been understood for a long time that factors like, complex forming
and protein binding greatly influence the bio-availability of compounds. The data is
normally better explained by extrapolating data using free instead of nominal
concentrations in tests. However, it has been shown that in some cases using free
concentration results in an under-estimation of the toxic effect. In this study it is reviewed
how and to what extent facilitated transport influences the accuracy of extrapolation
methods. It will be explained how facilitated transport is defined and how the theory
originally designed for metal complexes might be extrapolated to other situations like
facilitated transport by proteins. Furthermore it will be discussed that the influence of
facilitated transport only has to be taken into account when certain criteria are met.
Firstly, there has to be an extracellular ligand for the agent of interest, second the
Davison criterium has to be met, thirdly the derived data should be influenced by
alterations in kinetics and the ligands should be present or absent in either the in vitro or
in vivo situation. | |
dc.description.sponsorship | Utrecht University | |
dc.format.extent | 614634 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.title | The relevance of the facilitated transport hypothesis for in vitro to in vivo extrapolation in toxicology. | |
dc.type.content | Master Thesis | |
dc.rights.accessrights | Open Access | |
dc.subject.keywords | facilitated transport | |
dc.subject.keywords | facilitated diffusion | |
dc.subject.keywords | IVIVE | |
dc.subject.keywords | in vitro extrapolation | |
dc.subject.keywords | bio-availability | |
dc.subject.keywords | free concentration | |
dc.subject.keywords | protein binding | |
dc.subject.keywords | in vitro toxicology | |
dc.subject.courseuu | Toxicology and Environmental Health | |