| dc.rights.license | CC-BY-NC-ND | |
| dc.contributor | Dr. Ramon van den Bos | |
| dc.contributor.advisor | Breukink, E.J. | |
| dc.contributor.author | O'Sullivan, Killian | |
| dc.date.accessioned | 2024-08-31T23:01:58Z | |
| dc.date.available | 2024-08-31T23:01:58Z | |
| dc.date.issued | 2024 | |
| dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/47550 | |
| dc.description.sponsorship | Utrecht University | |
| dc.language.iso | EN | |
| dc.subject | A novel protein, natamycinase, is secreted by strains of Penicillium discolor and allows the host to grow on the surface of hard cheeses despite the presence of natamycin, a ubiquitous polyene food-preservative. Natamycinase cleaves natamycin thus removing its anti-fungal effects, thereby giving polyene resistance to its host. This thesis recombinantly expressed natamycinase in two heterologous hosts and attempted to purify the protein. | |
| dc.title | Recombinant Expression and Purification of Natamycinase: A Novel Serine Esterase Capable of Degrading the Polyene Antibiotic Natamycin | |
| dc.type.content | Master Thesis | |
| dc.rights.accessrights | Open Access | |
| dc.subject.keywords | Penicillium discolor; Esterase; Aspergillus niger; Protein Purification; Escherichia coli; Antibiotic Resistance; Food Spoilage; Heterologous Expression | |
| dc.subject.courseuu | Bio Inspired Innovation | |
| dc.thesis.id | 17494 | |
