Subtomogram averaging reveals in situ structure of dimeric hibernating ribosomes in archaea
| dc.rights.license | CC-BY-NC-ND | |
| dc.contributor.advisor | Förster, Friedrich | |
| dc.contributor.author | Schijndel, Vera van | |
| dc.date.accessioned | 2023-07-31T23:00:49Z | |
| dc.date.available | 2023-07-31T23:00:49Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/44425 | |
| dc.description.sponsorship | Utrecht University | |
| dc.language.iso | EN | |
| dc.subject | We investigated hibernating ribosomes from the hyperthermophilic archaeon Pyrococcus furiosus in situ using cryo-electron tomography (cryoET). Using an advanced subtomogram averaging workflow, we show that approximately 10% of ribosomes form dimeric structures in the stationary phase. We demonstrate that dimeric ribosomes are in a single conformation, while monosomes adopt various conformations. In summary, we revealed the first native hibernating ribosome dimer structure from archaea. | |
| dc.title | Subtomogram averaging reveals in situ structure of dimeric hibernating ribosomes in archaea | |
| dc.type.content | Master Thesis | |
| dc.rights.accessrights | Open Access | |
| dc.subject.courseuu | Molecular and Cellular Life Sciences | |
| dc.thesis.id | 7148 |