| dc.rights.license | CC-BY-NC-ND | |
| dc.contributor.advisor | Förster, Friedrich | |
| dc.contributor.author | Spoelstra, Marèl | |
| dc.date.accessioned | 2023-05-24T23:00:49Z | |
| dc.date.available | 2023-05-24T23:00:49Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/43920 | |
| dc.description.sponsorship | Utrecht University | |
| dc.language.iso | EN | |
| dc.subject | We optimized the purification strategies of large macromolecular complexes in the hyperthermophile P. furiosus. This thesis describes the optimization we did, the biochemical strategies used and the data analysis performed. A major part of this thesis is focused on the crude purification methods, whereby the complexes were purified in silico after cryo-EM images were taken of grids containing a mixture of several macrocomplexes. Molecules of interest were the 50S ribosome and encapsulins. | |
| dc.title | Purification of Large Protein Complexes in P. furiosus using Crude Purification Methods | |
| dc.type.content | Master Thesis | |
| dc.rights.accessrights | Open Access | |
| dc.subject.keywords | Macromolecular Complexes, Ribosome, Proteasome, Crude Isolation, Sucrose Gradient, VLPs, Encapsulin,
Archaea, Pyroccocus furiosus, Cryo-Electron Microscopy | |
| dc.subject.courseuu | Molecular and Cellular Life Sciences | |
| dc.thesis.id | 8435 | |
