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dc.rights.licenseCC-BY-NC-ND
dc.contributorN.v.t.
dc.contributor.advisorVader, Pieter
dc.contributor.authorSievers, Eva
dc.date.accessioned2023-03-15T01:01:03Z
dc.date.available2023-03-15T01:01:03Z
dc.date.issued2023
dc.identifier.urihttps://studenttheses.uu.nl/handle/20.500.12932/43651
dc.description.abstractThe use of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have gained much attention because of their therapeutic potential for cardiac repair. However, their translation to the clinic is dependent on overcoming challenges, such as large-scale extracellular vesicle (EV) production. Although serum starvation is a widely used technique to increase EV production, it remains insufficient for large-scale production. On that note, a chemically defined medium for high density cell culture (CDM-HD) is believed to increase MSC-EV production in hollow fiber bioreactors; however, no comparative analysis has been performed between EVs from CDM-HD-cultured MSCs and starved MSCs in 2D cell culture systems. Therefore, this project aimed to assess the effects of culture conditions with CDM-HD on the production of human fetal MSC-EVs, and whether these EVs are functional. For the comparative analysis, EV yields from MSCs cultured in Opti-MEM or MEM-α with 10% CDM-HD supplementation or without (starvation) were determined using nanoparticle tracking analysis and western blot analysis. EV functionality was assessed using a scratch wound assay. The results showed that when compared to their starvation counterpart, Opti-MEM with CDM-HD (OM+CDM) resulted in the highest EV yield with a 9.6-fold increase within 24h, whereas within 48h, MEM-α with CDM-HD (M+CDM) increased EV yield with 17.2-fold. Regarding EV functionality, M+CDM EVs were able to induce endothelial cell migration; however, minor differences were observed with the procedural control. Taken together, culturing with CDM-HD increases EV yield to a greater extent than serum starvation. However, M+CDM EV functionality should be further explored to realize its potential as therapeutic agent for cardiac repair.
dc.description.sponsorshipUtrecht University
dc.language.isoEN
dc.subjectThe assessment of the effects of culture conditions with Chemically Defined Medium for High Density cell culture on the production of human fetal mesenchymal stem cell-derived extracellular vesicles, and whether these EVs are functional.
dc.titleImpact of chemically defined media conditions on the production and regenerative function of mesenchymal stem cell‐derived extracellular vesicles
dc.type.contentMaster Thesis
dc.rights.accessrightsOpen Access
dc.subject.keywordsExtracellular vesicle production, mesenchymal stem cells, culture media formulation, cardiac repair
dc.subject.courseuuScience and Business Management
dc.thesis.id14933


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