dc.rights.license | CC-BY-NC-ND | |
dc.contributor.advisor | Externe beoordelaar - External assesor, | |
dc.contributor.author | Muhamad Rifki Ramadhan, Ramadhan | |
dc.date.accessioned | 2022-08-04T23:00:44Z | |
dc.date.available | 2022-08-04T23:00:44Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/42134 | |
dc.description.abstract | Milk is a resource of lipids, proteins, amino acids, vitamins, and minerals.
Proteins in milk vary in biological activities which include among others
antimicrobial, nutrients absorption facilitator, growth factors, hormones,
enzymes, and antibodies. Milk proteins also exist in various isoforms which
may differ in their activity. However, we still do not know much about the
variation of milk proteins other than the major ones (the caseins, β-
lactoglobulin and α-lactalbumin). The traditional methods of protein
analysis (e.g. 2D PAGE) do not suffice to study minor milk proteins which
comprise a very little fraction of milk protein. This project aims to find new
milk protein variants in Dutch milk using genomic approach. DNA from 54
Dutch Holstein bulls are sequenced and called for variants. Variants effects
on protein are predicted and protein variants from approximately 400 milk
proteins are determined. Variants found in 209 proteins including the known
variants of major milk proteins. Based on in silico protein analysis, ten
variants are listed as the most interesting variants, including milk protein
4. LC-MS analysis confirms the presence of a milk protein 4 variant. | |
dc.description.sponsorship | Utrecht University | |
dc.language.iso | EN | |
dc.subject | In this research, we performed DNA variant mining to find new milk protein variants in Dutch milk. We also did some protein in silico analysis to predict the structure and functionality of these new variants. | |
dc.title | DNA Variant Mining and In silico Analysis of Milk Proteins | |
dc.type.content | Master Thesis | |
dc.rights.accessrights | Open Access | |
dc.subject.keywords | variant mining; WGS; milk; genomics | |
dc.subject.courseuu | Bioinformatics and Biocomplexity | |
dc.thesis.id | 7706 | |