dc.rights.license | CC-BY-NC-ND | |
dc.contributor.advisor | Galac, Drs. S. | |
dc.contributor.advisor | Mol, J.A. | |
dc.contributor.author | Arts, D.P. | |
dc.date.accessioned | 2009-09-24T17:00:47Z | |
dc.date.available | 2009-09-24 | |
dc.date.available | 2009-09-24T17:00:47Z | |
dc.date.issued | 2009 | |
dc.identifier.uri | https://studenttheses.uu.nl/handle/20.500.12932/3543 | |
dc.description.abstract | Introduction : Hyperadrenocorticism is a common disease in dogs by which adrenocortical cortisol secretion is increased. One of the causes of hypercortisolism is a functional adrenocortical tumor. Adrenocorticotropic hormone (ACTH) is a major regulator in steroid synthesis. Human research has revealed a high ACTH-receptor mRNA expression in functional adenomas and low expression in carcinomas compared to controls. This research compared the expression of the ACTH-receptor in the adrenal cortex of 39 dogs with adrenal-dependent hypercortisolism, of which 27 are carcinomas and 12 are adenomas, with 15 normal adrenals.
Materials and methods: Adrenal tissue was derived from 39 dogs with hypercortisolism (27 carcinomas and 12 adenomas) and 15 normal adrenals served as controls. ACTH-receptor mRNA levels are measured with quantitative PCR, while ACTH-receptor protein levels are examined by western blot.
Results: ACTH-receptor mRNA expression is downregulated in carcinomas compared to normal adrenals. Expression in adenomas is not significantly different from controls. Western blot results show a downregulation of ACTH-receptor expression and therefore support the qPCR results. | |
dc.description.sponsorship | Utrecht University | |
dc.format.extent | 361509 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.title | Expression of the adrenocorticotropin receptor in cortisol-secreting adrenocortical tumors and healthy adrenals in dogs | |
dc.type.content | Doctoral Thesis | |
dc.rights.accessrights | Open Access | |
dc.subject.keywords | ACTH-receptor, adrenal, hypercortisolism, dog | |
dc.subject.courseuu | Diergeneeskunde | |