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dc.rights.licenseCC-BY-NC-ND
dc.contributor.advisorEgberink, Herman
dc.contributor.authorCornelissen, A.B.
dc.date.accessioned2018-09-25T17:01:46Z
dc.date.available2018-09-25T17:01:46Z
dc.date.issued2018
dc.identifier.urihttps://studenttheses.uu.nl/handle/20.500.12932/32332
dc.description.abstractMultiple pathogens of cats are directly associated with clinical abnormalities or have primary importance as zoonotic agents impacting human health. However, limited or no prevalence data is available for many of these pathogens in cats from the Netherlands. The aims of this study were therefore to estimate the prevalence of selected bloodborne and zoonotic pathogens in cats from the Netherlands and to assess for associated risk factors and co-infections. Anti-coagulated blood, sera, and or plasma were obtained from 167 client-owned cats in the Netherlands and transported to Colorado for assay. Previously validated plate based ELISA assays were used to detect antibodies against Bartonella species and Toxoplasma gondii in plasma or serum. A commercially available kit was used to detect antibodies against feline immunodeficiency virus (FIV) and antigens of feline leukemia virus (FeLV) and Dirofilaria immitis (SNAP® feline Triple; IDEXX) in sera or plasma. Total DNA and RNA were extracted from the blood and previously validated molecular assays that amplify the nucleic acids of Anaplasma species, Bartonella species, Ehrlichia species, Felis catus gammaherpesvirus 1 (FcaGHV1), feline foamy virus (FFV) and the hemoplasmas were performed. Genetic sequencing was performed to determine the species of some agents and to validate the final results. The estimated seroprevalence rates for Bartonella spp. IgG (21 of 163 cats; 12.9%; 95% CI: 8.51-19.02), T. gondii IgG (26 of 163 cats; 16.0%; 95% CI: 11.05-22.48), FIV antibodies (6 of 121 cats; 5.0%; 95% CI: 2.21-10.71) and FeLV (4 of 121 cats; 3.3%; 95% CI: 1.23-8.59) were calculated. Bartonella spp. DNA (2 of 163 cats; 1.2%; 95% CI: 0.30-4.83), overall hemoplasma DNA (17 of 163 cats; 10.4%; 95% CI 6.55-16.21), FFV DNA (66 of 150 cats; 44.0%; 95% CI: 36.19-52.12) and FcaGHV1 DNA (21 of 162 cats; 13.0%; 95% CI: 8.57-19.14) were amplified from some samples. Risk factors included increasing age for FFV infection and co-occurrences of infection were found for several pathogens. D. immitis antigen as well as Anaplasma spp. and Ehrlichia spp. DNA were not detected in any of the 121 and 163 samples tested respectively.
dc.description.sponsorshipUtrecht University
dc.language.isoen_US
dc.titlePrevalence of infections with selected pathogens in client-owned cats from the Netherlands
dc.type.contentMaster Thesis
dc.rights.accessrightsOpen Access
dc.subject.keywordsFeline, Bartonella spp., Toxoplasma gondii, FIV, FeLV, Anaplasma spp., Ehrlichia spp., FcaGHV1, FFV, hemoplasma
dc.subject.courseuuGeneeskunde van gezelschapsdieren


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