Effects of NEFA's on Cell Viability and Progesterone Production of Bovine Corpus Luteum Cells

Abstract

Bovine corpus luteum cells were cultured for 6 days. On day 6 medium was collected and NEFA’s (100 and 250 µM of oleic and palmitic acid) were added. In the medium progesterone analysis is performed. Cells were fixated and colored using immunofluorescence. Images were made on Leica Confocal fluorescence microscope and cells were counted. 100 µM of oleic acid compared to 100 µM BSA and 100 and 250 µM palmitic acid compared to control group without BSA showed a negative effect on cell viability. Progesterone production per 100 cells showed no significant difference, however a trend is seen that the production per 100 cells increased when NEFA’s were added.