Validatie Precision Xceed met gehepariniseerd runderbloed
Gorissen, Willem Jan
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Abstract The goal of this study was to validate a new cowside test for measuring β-hydroxybutyrate in bovine heparin plasma, the Precision Xceed. Therefore, blood samples from 24 lactating cows were tested and compared with a laboratory test. The values from the cowside test correlated well with the values from the laboratory (R = 0.98). Sensitivity en specificity were 100 en 94.4 %, using a subclinical ketosis threshold of > 1200µmol BHBA/L. A parallelly conducted pilot with 4 plasma samples indicated that within 24 hours and with different temperatures, the concentration of BHBA was hardly altered. Also paired data from an urine nitroprussid test and the BHBA concentration in the same urine were analyzed. A given outcome from the nitroprussid test resulted in a rather dispersed concentration of BHBA. As a topping, options for a cowside diagnosis of (sub)clinical ketosis were investigated in literature. This in particular to determine how to estimate the concentration of BHBA in plasma with a given outcome from a urine nitroprussid test.